Effect of TQ on cell viability and apoptosis in oral cancer cells.

<p>(A) SCC-4, SAS, SASVO3, OC2, and (B) S-G cells were treated with various TQ concentrations for 24 h. Cell viability was analyzed by MTT assay. (C) SASVO3 cells were treated with TQ for 24 h. Viable cells were then collected and counted using a hemocytometer. (D) Equal numbers of SASVO3 cells were plated and stained using colony formation assay as described in the text. (E) SASVO3 cells were treated with TQ (0, 20, 40, and 60 µM), and cell cycle distributions were assessed by flow cytometry using PI staining. (F) Flow cytometry analysis of annexin V/PI double staining was conducted to determine the number of apoptotic cells. The statistical significance of the results was analyzed by one-way ANOVA and post hoc Dunnett's test (^*<i>p</i><0.05, ^**<i>p</i><0.01, ^***<i>p</i><0.001).