Effects of ET antagonists on induction of GFAP-positive astrocytes by cold injury (Protocol 2).

<p>ET antagonists (10 µg) were repeatedly administered at 12-h intervals from 24 h after cold injury. Mouse brains were fixed at 72 h after cold injury. GFAP-positive astrocytes in the injured and the contralateral hemisphere were counted. Representative photographs of the injured hemisphere are shown. Scale bar = 50 µm. Results are means ± SEM of six experiments. **p<0.01 vs. Sham-operated group, ^##p<0.01 vs. vehicle administration by one-way ANOVA followed by the Tukey’s test.