Effects of ET antagonists on brain water content (Protocol 1: Administration prior to cold injury).

<p><b>(A) Comparison of the effects of ET antagonists.</b> Ten µg of BQ788, IRL-2500, FR139317, or bosentan was injected into the right cerebroventricle 30 min before cold injury. Mouse brains were removed at 6 h or 12 h after cold injury. Water content in the injured and the contralateral hemisphere was measured. Results are means ± SEM of six to eight experiments. **p<0.01 vs. Sham-operated group, ^##p<0.01 vs. vehicle administration by one-way ANOVA followed by the Tukey’s test. <b>(B)</b><b>Dose-dependent effects of ET antagonists.</b> BQ788, IRL-2500, FR139317 and bosentan at the dosages indicated were administered 30 min before cold injury. Water content in the injured and the contralateral hemisphere was measured at 12 h after cold injury. Results are means ± SEM of five experiments. **p<0.01 vs. Sham-operated group, #p<0.05, ##p<0.01 vs. vehicle administration by one-way ANOVA followed by the Tukey’s test.