Induction of reactive astrocytes after cold injury in mouse cerebrum.

<p>(<b>A</b>) Representative photographs showing GFAP-positive reactive astrocytes in the injured and contralateral areas 12 h after cold injury. Induction of reactive astrocytes was observed in sagittal brain sections positioned 4–6 mm from bregma. These sections contained peri-core regions around the core areas damaged by cold injury. Scale bar = 200 µm. (<b>B</b>) Time-course of the increase in GFAP-positive cells in the injured regions. GFAP-positive cells in the injured and the contralateral hemisphere were counted at the times indicated after cold injury. The numbers of GFAP-positive cells are shown as numbers of positive cells per mm² of observed area. Results are means ± SEM of six experiments. **p<0.01 vs. 0 h by one-way ANOVA followed by the Dunnett’s test.