Transmigration of wild type (THP-1 WT), empty plasmid (Control) and ACE-overexpressing THP-1 monocytes (ACE1, ACE2, ACE3).

<p>(A) Transmigration of calcein-labelled cells through membrane towards (A) medium supplemented with MCP-1 or (B) HUVEC monolayers in the presence of medium only. (C, D, E) Transmigration of the cells through endothelial monolayers under MCP-1. See representative images (D, E). Analyses were performed in 10 random microscopic fields each. Means ± SD of cell number in 10 microscopic fields in three independent experiments. (F, G) Expression of MCP1 and CCR2 by RT-PCR and FACS analysis respectively. * p< 0.05 vs. control indicates statistical significance. Means ± SD of three independent experiments.