Expression of ACE and behaviour of human monocytes under uremic status II.

<p>(A, B) Primary human monocytes obtained from healthy volunteer P1 were treated with (A) 10% NS or PD or CKD5 or (B) 10% NS or pre- or post-HD sera for 72 h and investigated for ACE-mRNA expression. Means ± SD of three independent experiments. * p< 0.05 (PD vs. NS; pre-HD vs. NS; pre-HD vs. post-HD) and °p<0.05 (CKD5 vs. NS; post-HD vs. NS) indicate statistical significance. (C, D, E, F) Adhesion of primary monocytes treated with NS or PD or CKD5 sera. Primary human monocytes were incubated in the presence of 10% NS or PD or CKD5 sera for 30 min and investigated for their endothelial-adhesion. The number (F) and corresponding images of endothelial-adhered monocytes (C, D, E) are shown. Means ± SD of cell number in 10 microscopic fields in three independent experiments. (G, H, I, J) Transmigration of calcein-labelled primary monocytes through endothelial monolayer towards lower chamber filled with RPMI medium supplemented with 10% NS or PD or CKD5 sera. Transmigrated cells were visualized with fluorescent microscopy (G, H, I) and counted in 10 random fields (J). Representative images are shown. Means ± SD of cell number in 10 microscopic fields in three independent experiments. * p< 0.05 indicates statistical significance.