Nuclear β-catenin presence during chick eye development.

<p>(A) Schematic diagram of the eye showing the anterior (A) and posterior (P) region of the eye as well as the location of the ciliary margin (CM), retina (R), retinal pigmented epithelium (RPE) and Lens (L). The orientation of the eye in the diagram applies to all images in this paper. (B–F) Immunohistochemistry showing the location of nuclear β-catenin (Nu β-cat) in the developing optic vesicles at E1.5 (HH 10) (B–D) and E2 (HH 13) (E–F). (G–P) Nu β-cat presence in the developing CM and RPE at E4 (HH 22–24) (G–K) and E5 (HH 27–28) (L–P). DAPI stains the nuclei of the cells in (B, D, F, G, I, K, L, N, P). B, G and L are the corresponding negative controls for E 1.5 (B), E4 (G) and E5 (L). SE: surface Ectoderm; NE: neuroepithelium; NPE: non-pigmented epithelium; PE: pigmented epithelium. OCL: optic cup lip. The scale bar in (B) represents 100 µm and applies to all panels.