Wholemount fluorescent microscopy of novel gonad promoter expressed EGFP.

<p>Tissues from E7.5 embryos infected with RCANBP viruses containing <i>SV40</i> (<i>SV40p</i>), <i>WT1</i> (<i>WT1p</i>), <i>SF1</i> (<i>SF1p</i>), <i>AMH</i> (<i>AMHp</i>) and <i>aromatase</i> (<i>AROMp</i>) promoters. Dashed white lines delineate the left (Lg) and right (Rg) gonads, which sit on top of the mesonephros (Ms). A: Strong EGFP expression was evident for <i>SV40p</i> and <i>AROMp</i>, however, this was not confined to the urogenital systems. <i>WT1p</i> and <i>AMHp</i> produced low-level expression in the urogenital systems. EGFP expressed from <i>SF1p</i> was moderate in the urogenital system, and included the gonads. B: RCANBP-SF1p-EGFP infected E7.5 embryo; in addition to EGFP expression in the urogenital system, embryos also showed EGFP expression in the liver, forelimb (FL) and hind limb (HL).