A: Quantitative RT-PCR confirmation of microarray data.

<p>Relative expression of the <i>Cpn2</i>, <i>Zfp353</i>, <i>Htr4</i>, <i>Nkx6-3</i> and <i>Xist</i> transcripts in the cerebral hemispheres of male newborn pups, from mothers supplemented with FA at 4 mg/kg of diet. The results were normalized to <i>Hprt</i> transcript expression, and expressed as relative values in comparison to corresponding transcripts from newborn pups with FA at 0.4 mg/kg diet. Results represent means ± S.D; asterisks denote statistically significant change (***<i>P</i><0.001, **<i>P</i><0.01 and *<i>P</i><0.05). <b>B</b>): <b>Quantitative RT PCR confirmation of microarray data</b>. Relative expression of the <i>Vgll2</i>, <i>Leprel1</i>, <i>Nfix</i> and <i>Slc17a7</i> transcripts from the cerebral hemispheres of female newborn pups, from mothers supplemented with FA at 4 mg/kg of diet. The results were normalized to <i>Gapdh</i> transcript expression, and were expressed as relative values in comparison to corresponding transcripts from newborn pups with FA at 0.4 mg/kg diet. Results represent means ± S.D; asterisks denote statistically significant change (***<i>P</i><0.001, **<i>P</i><0.01 and *<i>P</i><0.05).