TALEN-induced frameshift alleles of <i>Pcnxl2</i> and suppression of <i>Gria4</i> SWD.

<p>A. Exon-intron structure of mouse <i>Pcnxl2</i> (from UCSC genome browser, version m38 mouse genome assembly), highlighting the position of the HeJ-specific IAP-1Δ1 insertion in intron 19, the two exons (exon 16 and exon 29, respectively) targeted for TALEN mutagenesis and the wildtype FeJ sequence of each together with the sequence of the respective frameshift alleles in TALEN mutations A+1 (FS2), A−11 (FS1), and B−2 (FS3). The asterisk indicates a premature translational stop codon in each mutant allele. B. Spike-wave discharge (SWD) incidence (top) and length (bottom) for parent FeJ-<i>Gria4</i>^IAP congenic and HeJ-<i>Gria4</i>^IAP inbred strain colonies, compared with littermate wildtype (wt), heterozygous or homozygous respective TALEN mutations created and maintained on the isogenic FeJ-<i>Gria4</i>^IAP congenic strain background. Asterisks indicate where homozygous mutant genotypes were significantly different (<i>p</i><0.01) from wildtype control. Sample sizes: FeJ-<i>Gria4</i>^IAP (9), HeJ- <i>Gria4</i>^IAP (18), wt (16), FS1 het (6), FS1 hom (6), FS2 het (9), FS2 hom (9), FS3 het (6), FS3 hom (6). Error bars are SEM. C. Spike-wave discharge (SWD) incidence (left) and length (right) for littermate TalA-11 genotypes (hom, het, wt) isogenic on the FeJ strain, in double mutant combination with <i>Gabrg2</i>^tm1Spet or <i>Scn8a</i>^8J/+ from a congenic FeJ strain background. None of the <i>Pcnxl2</i> mutant allele homozygotes were significantly different from the other <i>Pcnxl2</i> genotypes for either mutation or SWD measure. Sample sizes: <i>Gabrg2</i>^tm1Spet (3 hom, 12 het, 2 wt); <i>Scn8a</i>^8J/+ (2 hom, 4 het, 2 wt).