GW4064 and CDCA reduce HDL endocytosis independently of SR-BI.

<p>(a) HepG2 cells were treated with the indicated concentrations of GW4064 or chenodeoxycholate (CDCA) in media containing lipoprotein-deficient serum (lpds) for 24 hours and gene expression was analyzed by qRT-PCR (n = 3). (b) Cells were incubated with 10 µM GW4064 or 100 µM CDCA in media containing lpds for 24 hrs and protein expression was determined by western blot analysis and results were quantitated by densitometry (n = 3). HepG2 cells transfected with scrambled shRNA (c) or SR-BI shRNA (d) were incubated with 10 µM GW4064 or 100 µM CDCA in media containing lpds for 24 hours. Cells were then incubated with 20 µg/ml double labeled ¹²⁵I/³H-CE-HDL for 1 hr. Selective cholesteryl-ester uptake was calculated by subtracting ¹²⁵I-HDL uptake from ³H-CE-HDL uptake (n = 3).