Intestinal Manipulation leads to decreased populations in the spleen but doesn’t affect the MLN.

<p>(A). The number of DCs, macrophages, B cells and CD8 T cells is not significantly altered 24 h after IM in the spleen whereas the number of CD4 T cells is significantly decreased. (B). The number of cells of all population measured remains identical in the MLN after L and IM. Data shown are mean ± SEM of 2 independent experiments (n = 8–9 animals per group). p<0.05 (Student t-test).