Intestinal manipulation doesn’t increase splenocyte cell death.

<p>A. Late apoptosis was determined in the spleen by a TUNEL immunofluorescent assay 24(in green) between L and IM groups. B. Necrotic (AnnexinV+ Live/dead marker+) and apoptotic cell death (AnnexinV+ Live/dead marker-) in the spleen were quantified by FACS analysis 24 h after IM. The percentage of necrotic and apoptotic cell death remains unchanged between L and IM mice. Data shown are mean ± SEM of 2 independent experiments (n = 4 animals per group) (Student t-test).