Attenuated airway resistance in sensitized <i>Ramp1^−/−</i> and <i>Calcrl^+/−</i> animals compared to wildtype.

<p><b>A... </b><i>Ramp1^+/+</i> and <i>Ramp1^−/−</i> animals were sensitized to ovalbumin (OVA) as described in the methods section prior to challenging the animals with either aerosolized saline (PBS) or ovalbumin (OVA) for five days. The maximum airway resistance (R_{AW max}) at the basal state and after challenge with aerosolized methacholine (MCh) was determined. (n = 3–4 per group) <b>B... </b><i>Calcrl^+/+</i> and <i>Calcrl^+/−­</i> animals were sensitized to ovalbumin prior to challenge as described in panel A. R_{AW max} was determined for the basal state and in response to MCh challenge 24 hours after challenge with aerosolized PBS or OVA. (n = 3–4 per group) <b>C... </b><i>Calcrl</i> was specifically knocked down in smooth muscle cells using the SM22-Cre mouse. These <i>SM22-Cre:Calcrl^fl/fl</i> mice and wildtype controls were then sensitized and challenged as described in panels 3A and 3B. (n = 4 per group) Data represent means±SEM.*p<0.05 <i>Ramp1^−/−</i> (OVA) compared to <i>Ramp1^+/+</i> (OVA), #p<0.05 <i>Calcrl^+/−</i> (OVA) compared to <i>Calcrl^+/+</i> (OVA).