Targeting vector for the generation of <i>Ramp1</i> knockout mice.

<p><b>A.</b> Targeting vector design for excision of exon 3 from the mouse <i>Ramp1</i> gene. (E = EcoRI, B = BamHI) <b>B.</b> Southern blot to confirm generation of <i>Ramp1</i> floxed allele. <b>C.</b> Southern blot confirming the successful generation of <i>Ramp1^+/−</i> after Cre-mediated excision of exon 3. <b>D.</b> Routine genotyping using primers 1 (P1) and 2 (P2) shown in panel 1A.