Expression of <i>35S-topp4-1</i>, <i>pTOPP4-topp4-1</i>, or <i>35S-TOPP4</i> in wild-type plants.

<p>(<b>A</b>) Expression of <i>35S-topp4-1</i> or <i>pTOPP4-topp4-1</i> in wild type recapitulates the dwarfed phenotypes of <i>topp4-1</i>. Five-week-old <i>35S-topp4-1</i> transgenic plants are shown on the left, and upper right corner is the higher magnitude picture of the #2 transgenic plant. Five-week-old <i>pTOPP4-topp4-1</i> transgenic plant is shown on the right. (<b>B</b>) qRT-PCR was used to detect relative expression levels of the <i>topp4-1</i> in three <i>35S-topp4-1</i> transgenic lines shown in (<b>A</b>). The expression level of <i>TOPP4</i> in wild type was set to 1.0. (<b>C</b>) Representative 4-week-old <i>amiR-TOPP4 topp4-1</i> transgenic lines. The first number 1 or 2 of the lines represents transgenic plants generated by <i>amiR-TOPP4</i>-1 <i>or amiR-TOPP4</i>-2, respectively. (<b>D</b>) qRT-PCR was used to detect the relative expression levels of <i>topp4-1</i> in three <i>amiR-TOPP4 topp4-1</i> transgenic lines shown in (<b>C</b>). The expression level of <i>topp4-1</i> in the <i>topp4-1</i> mutant was set to 1.0. (<b>E</b>) qRT-PCR was used to detect the relative expression levels of <i>TOPP4</i> in seven 2-week-old transgenic seedlings overexpressing <i>TOPP4</i>. The expression level in wild type was set to 1.0. (<b>F</b>) Five-week-old transgenic plant overexpressing <i>TOPP4</i> shows increased inflorescence and enlarged organ size compared to the wild-type plant. One representative line (#7) is shown. Three-week-old wild-type plant (left) and <i>35S-TOPP4</i> #7 (right) are shown in the upper left corner. Scale bars = 1 cm in (<b>A</b>) and (<b>F</b>), 0.5 cm in (<b>C</b>). Asterisks in (<b>B</b>), (<b>D</b>) and (<b>E</b>) represent statistic differences based on Student's <i>t</i> test with P<0.05. In (<b>B</b>), (<b>D</b>) and (<b>E</b>), error bars represent SE (n = 3).