PGC depletion in <i>Fancm^C4/C4</i> mice is associated with reduced proliferation, not apoptosis.

<p>(A) Male and female embryonic gonads from E11.5 and E12.5 stained for alkaline phosphatase activity. A decrease in PGCs is becomes evident only at the latter time point. The graphs of germ cells in (B) represent quantification of germ cells by immunolabelling fetal gonads with either Stella (E11.5 and E12.5) or MVH (E13.5). Representative images for E13.5 gonads are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004471#pgen.1004471.s002" target="_blank">Figure S2</a>. The graphs of BrdU+ cells represent data from BrdU incorporation assays shown in (C and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004471#pgen.1004471.s002" target="_blank">Figure S2</a>). BrdU and Stella double-positive cells, which represent PGCs in S phase, were quantified as percentage of total Stella-positive PGCs.