Melanocyte rescue occasionally occurs at a distance from the clone boundary in skin clones.

<p>A-A’’. While the majority of clones expressing <i>kitlga</i> only cause melanocyte rescue within the clone boundary (yellow line), some skin clones promote additional melanocyte rescue at a distance >1 fin ray (individual grey arrowheads. A’. Rogue labeled epidermal cells (arrows) along the edges of clonal boundaries may separate from the bulk of the clone mass and intercalate with unlabeled epidermal populations, supporting limited melanocyte regeneration at a distance. B. Quantification of the ability of different clone lineages to rescue melanocytes at significant distances from the clonal boundary. Brackets show the proximal-distal extent of melanocyte rescue within the clone. Black arrowheads indicate previously differentiated kit-independent melanocytes from the stump. Grey arrowheads indicate newly differentiated melanocytes. White arrows indicate GFP+ cells that are outside of the contiguous regenerated skin clone. Red dashed lines indicate the amputation plane. Scale bar = 250 mm.