Overexpression of miR-203 inhibited of TGF-β1-induced HSC proliferation.

<p>A. Downregulation of miR-203 expression in liver fibrotic tissues compared with vehicle-treated groups. The miR-203 expression was analyzed by one-step quantitative real-time PCR. *P<0.05 vs vehicle control. B. Downregulation of miR-203expression in response to TGF-β1 (0, 5, 10 ng/ml) for 24 h. The miR-203 expression was analyzed by one-step quantitative real-time PCR. **P<0.01 vs non-treated cells. C. Upregulation of miR-203 expression in transfected HSC. The miR-203 expression of HSC was analyzed by one-step quantitative real-time PCR. **P<0.01 vs NS-miRNA. D. MiR-203 overexpression significantly inhibited proliferation of TGF-β1-induced HSC. The role of miR-203 in regulating TGF-β1-treated HSC proliferation was tested by MTT assay. The data represent the mean±SD of three different experiments. *P<0.05 vs control(non-treated cells), ^#P<0.05 vs NS-miRNA.