Infiltrating myeloid cells display reduced expression of surface maturation markers during murine NCC.

<p><i>In-situ</i> IF staining was performed on frozen sections of HBSS infected (mock) and parasite infected brains of mice at 1 wk and 2 wk p.i. Brain cryosections were analyzed for expression of maturation markers MHC II, CD80, and CD86 using fluorochrome conjugated antibodies (red-PE) and macrophages specific marker CD11b (green-Alexa 488). Nuclear staining DAPI is depicted in blue. Staining for these maturation markers MHC-II (A1), CD80 (B1), and CD86 (C1) was undetected/barely detected in mock infected mice. Parasite infected mice brains displayed large number of infiltrating myeloid cells stained positive for CD11b (A2–A3, B2–B3, and C2–C3). MHC-II was undetected in many of the CD11b+ accumulated cells in the CNS of NCC mice at both 1 wk (A2) and 2 wk p.i. (A3) (arrow). CD80 (B2–B3) and Cd86 (C2–C3) were undetected/barely detected in the CD11b+ myeloid cells around the parasite (P) in the CNS of NCC mice both at 1 wk and 2 wk p.i. Results are from one representative experiment of atleast three independent experiments (n = 4 per each time point).