α₅ Integrins Regulate HT1080 Fibrosarcoma Cell Remodeling Under Shear.

<p>(A) Coverslips were washed at the indicated times to eliminate unbound HT1080 fibrosarcoma cells, and the remaining cell density, normalized to untreated (WT) and unwashed controls at the indicated time points, is plotted for the indicated treatments. (B) Cell attachment strength to fibronectin was tested under the indicated conditions. Note that each representative curve represents thousands of cells grouped at set radial distances with data expressed as mean ± standard deviation. (C) Average attachment strength (T₅₀) for cells in the indicated conditions. (D) Fluorescent images of cells stained after application of the indicated shear for nuclei (DNA; blue) and actin (red). A white arrow indicates shear direction and open arrowheads indicate remodeled cells. Dashed lines indicate the regions of inset images, which are outlined in white. Note that all statistical analyses used non-parametric Kruskal-Wallis analysis of variance from triplicate experiments. ***p<0.001.