<p>Culture supernatants were collected 24-2 and the cytokines concentrations were specifically determined by ELISA. All data are expressed as mean ± SEM of three individual experiments (n = 12). Differences between the treated and untreated control groups were compared using one-way ANOVA. * P<0.05, ** P<0.01, *** P<0.001 for difference from culture without treatment.</p
<p>PBMC isolated from patients with chronic Chagas' disease Cardiomyopathy (CCC; n = 10) and non-inf...
<p>PBMC (2×10<sup>5</sup>/well) were exposed to 6×10<sup>5</sup> unopsonised IE or IE opsonised with...
<p>PBMC (2×10<sup>5</sup>/well) obtained from primigravid and multigravid women were exposed to 6×10...
Individual cytokine values from PBMCs stimulated with UP 39114, GBP 39113 and 39117 and P. vivax lys...
Individual data shows the mean value of non-stimulated and PBMCs stimulated with UP 39114, GBP 39113...
<p>PBMC were isolated from 6 healthy donors and cultured at 10<sup>6</sup>/ml for 5 days with UV-ina...
<p>(A),TNF-α levels were measured by ELISA at 4 h, 8h and24 h; (B), IL-6 levels were measured at 4h,...
<p>IL1-β, TNF-α and IL-6 levels measured by the 3-plex Luminex xMAP<sup>®</sup> assay in cell lysate...
<p>(A) IL-6, (B) IL-17, (C) IFN-γ, (D) IL-2 and (E) TNF-α levels measured by ELISA in supernatant fr...
Cytokine concentrations in cell culture supernatants were determined by ELISA for PBMCs infected wit...
<p>Cytokines were evaluated by magnetic bead-based multiplex immunoassays. The analysis was performe...
<p>Co-culture supernatants were harvested and assayed at different time points for IL-2, IFNγ, IL-6 ...
<p>Control wells were seeded with cells without any antigen/mitogen (Med-SN). The levels of (A) IL-...
<p>PBMC (2×10<sup>5</sup>/well) were exposed to 6×10<sup>5</sup> unopsonised IE or IE opsonised with...
<p>BM cells were cultured in 96-well plates for 6 days with each of 60 different cytokines (10 ng/ml...
<p>PBMC isolated from patients with chronic Chagas' disease Cardiomyopathy (CCC; n = 10) and non-inf...
<p>PBMC (2×10<sup>5</sup>/well) were exposed to 6×10<sup>5</sup> unopsonised IE or IE opsonised with...
<p>PBMC (2×10<sup>5</sup>/well) obtained from primigravid and multigravid women were exposed to 6×10...
Individual cytokine values from PBMCs stimulated with UP 39114, GBP 39113 and 39117 and P. vivax lys...
Individual data shows the mean value of non-stimulated and PBMCs stimulated with UP 39114, GBP 39113...
<p>PBMC were isolated from 6 healthy donors and cultured at 10<sup>6</sup>/ml for 5 days with UV-ina...
<p>(A),TNF-α levels were measured by ELISA at 4 h, 8h and24 h; (B), IL-6 levels were measured at 4h,...
<p>IL1-β, TNF-α and IL-6 levels measured by the 3-plex Luminex xMAP<sup>®</sup> assay in cell lysate...
<p>(A) IL-6, (B) IL-17, (C) IFN-γ, (D) IL-2 and (E) TNF-α levels measured by ELISA in supernatant fr...
Cytokine concentrations in cell culture supernatants were determined by ELISA for PBMCs infected wit...
<p>Cytokines were evaluated by magnetic bead-based multiplex immunoassays. The analysis was performe...
<p>Co-culture supernatants were harvested and assayed at different time points for IL-2, IFNγ, IL-6 ...
<p>Control wells were seeded with cells without any antigen/mitogen (Med-SN). The levels of (A) IL-...
<p>PBMC (2×10<sup>5</sup>/well) were exposed to 6×10<sup>5</sup> unopsonised IE or IE opsonised with...
<p>BM cells were cultured in 96-well plates for 6 days with each of 60 different cytokines (10 ng/ml...
<p>PBMC isolated from patients with chronic Chagas' disease Cardiomyopathy (CCC; n = 10) and non-inf...
<p>PBMC (2×10<sup>5</sup>/well) were exposed to 6×10<sup>5</sup> unopsonised IE or IE opsonised with...
<p>PBMC (2×10<sup>5</sup>/well) obtained from primigravid and multigravid women were exposed to 6×10...