L6 33K and 22K.

<p>(<b>A</b>) <i>Sequence of 33K and 22K</i>. The nucleotide numbers of BAdV-3 genome shown are according to Gene bank accession # AF030154. The changed nucleotide residues in 33K and 22K are shown in bold. The substituted amino acid in 33Ksr or 22Kw is shown in bold and underlines. (<b>B</b>). <i>Schematic representation of plasmid pFBAV33Ksr</i>. BAdV-3 genome (dotted pattern box). The thin line represented deleted E3 region <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0101216#pone.0101216-Zakhartchouk1" target="_blank">[45]</a>. The substituted amino acids (glycines) of 33K in BAdV-3 genome are underlined. The numbers represent the amino acid numbers of 33K. CMV (human cytomegalovirus immediate early promoter); EYFP (enhanced yellow fluorescent protein). Arrows represent the direction of transcription. The coding sequences shared by 33K and 22K (box with pattern) or specific for 33K (hollow box) and 22K (black box) are depicted. The spliced region in 33K is represented by dots flanked by splice donor\acceptor sites. The wild-type (GT…AG) and mutated (GC…CG) splice acceptor/donor sites are depicted. The numbers of the top denote amino acid numbers. (<b>C</b>) <i>Complementation of pFBAV33Ksr genome</i>. The VIDO DT1 cells were co transfected with indicated plasmids and the fluorescent focus forming units were counted at indicated days post transfection. The numbers on the X- axis denote the days post transfection.