Sub cellular localization of 33K proteins.

<p>(<b>A</b>) <i>Schematic representation of BAdV-3 33K fusion proteins</i>. The number above the box denote the amino acid number for 33K protein. Thick box represent BAdV-3 DNA; hollow box represents EYFP DNA; stripped box represents GFP DNA; dotted box represents β-gal DNA. Thin lines represent deleted regions. The name of the individual mutant protein is indicated on the left of the panel. The name of the individual plasmid is indicated on the right of the panel. (<b>B</b>). <i>Immunofluorescence</i>. Monolayers of MDBK cells (panel A) infected with BAdV-3. At 24 hrs post infection, the cells were analysed by indirect immunofluorescence by staining with anti-33Kp serum, Cy 2 conjugated goat anti-rabbit serum and DAPI. Similarly, monolayers of HeLa cells (panels B to P) were transfected with individual plasmid DNA expressing indicated 33K fusion proteins and analysed at 48 hrs post transfection. Finally, the cells were stained with DAPI and analyzed by direct fluorescence. The name of the protein is indicated on the right of the panel.