STAT6 is important for stable c-FLIP_S expression in Th2 cells.

<p>A. Thp cells were isolated from cord blood and activated (Th0) or also stimulated with IL-12 (Th1) or IL-4 (Th2) and samples for western blotting were collected at the indicated time-points. The panels show representative data from three independent biological replicate cultures. B. Bars represent the mean values (±SEM) of the relative levels of c-FLIP_S protein, obtained by quantifying and normalizing against the levels of β-ACTIN. The values of the Th0 samples were set as 1. Results were calculated from three independent biological replicate cultures. C. Freshly isolated Thp cells were transfected with STAT6 (S6) or non-targeting (NT) siRNA and polarized in Th0 or Th2 direction 20–24 h after transfection. Samples for western blotting were harvested at the indicated time-points. The panels show representative data of three biological replicate cultures. D. Bars represent the mean values (±SEM) of the relative levels of c-FLIP_L (upper panel) and c-FLIP_S (lower panel), obtained by quantifying and normalizing against the levels of GAPDH. The values of Th0 (24 h) and Th2 (24 h) were set as 1 and other Th0 and Th2 samples were compared to them. Results were calculated from three biological replicate cultures. Statistical significance was calculated using the paired student's t-test, * p>0.05. SEM = standard error of mean.