Rb regulates mitochondrial membrane proteins expression.

<p>(A) mRNA and protein levels of Bax, Bak and Bad in 3T3/wt and 3T3/Rb^−/− cells were determined by RT-PCR and western blot. The number indicates quantified ratio relative to the inter-control GAPDH or β-Actin. (B) Bad protein levels in 3T3/Rb^−/− cells with or without Rb re-expression. The number indicates quantified ratio relative to the inter-control β-Actin. (C) Bcl-XL was over-expressed in 3T3/Rb^−/− cells. (D) FL2/FL1 ratios were determined based on JC-1 fluorescence after 3T3/Rb^−/− cells with or without Bcl-XL over-expression were cultured for 24 h. (E) ROS levels were determined based on DCF fluorescence after 3T3/Rb^−/− cells with or without Bcl-XL over-expression were cultured for 24 h. *<i>P</i><0.05, **<i>P</i><0.01.