MAP2K4 overexpression specifically alters HSP27 phosphorylation and total protein expression.

<p>The expression of total and phosphorylated forms of HSP27 proteins were assessed by Western blot in the indicated cell lines, <b>A-B</b>. Data from three separate blots are graphically depicted in<b>B</b>, as mean ± SEM. <b>C</b>), HSP27 transcript levels were measured by qRT/PCR, normalized to GAPDH, and expressed as the mean ± SEM percentage of VC. Data are from three independent experiments, each in replicates of N = 2. * denotes p≤0.05 between the indicated groups.