Alignment of amino acid sequences of CmCADs.

<p>Conserved important regions identified previously are marked as follows: white arrows denotes catalytic zinc ion coordinating residue, black arrows denotes structural Zn ion coordinating residue, the black circle denotes key residues for substrate specificity. The black square denotes key Phe299/Gly(300) residues for substrate specificity. The white square denotes key Trp199 and Asp123 residues for substrate binding. Locations of the Zn1, Zn2, and NADPH binding domains are shown in boxes. The alignment was performed with the ClustalW2 software program.