Wnt5a increases MFU number independently of canonical Wnt signaling.

<p>Dissociated cells were treated as indicated and secondary mammospheres were counted one week after plating. (A) Numbers of wild-type and MMTV-<i>Wnt1</i> secondary mammospheres after treatment with or without 200 ng/ml Wnt5a. (B) Wild-Type mammospheres treated with Wnt3a, Wnt5a or in combination. Wnt3a or Wnt5a were used individually at 400 ng/ml while in combination each was applied at 200 ng/ml. (C) Wild-type secondary mammosphere numbers after treatment with recombinant Wnt5a, Dkk1, or both (200 ng/ml each). In panels A and B all values were significantly different from one another at p<.01; in C, treatments with and without Wnt5a were significantly different at p<.01. Error bars show 95% confidence intervals.