Distribution and localization of SERPINB12 mRNA and protein in normal and cancerous ovaries from laying hens.

<p>[A] Quantitative PCR was conducted using cDNA templates from normal and cancerous ovaries of hens. The expression of <i>SERPINB12</i> was unique to cancerous ovaries of laying hens as compared with normal ovaries from laying hens. [B] <i>In situ</i> hybridization analyses indicated cell-specific localization of <i>SERPINB12</i> mRNA in normal and cancerous ovaries from laying hens. <i>SERPINB12</i> was strongly expressed in the GE of cancerous ovaries whereas there was no expression in any cells of normal ovaries. [C] Immunoreactive SERPINB12 protein, as for <i>SERPINB12</i> mRNA, was also unique to the GE of cancerous ovaries from laying hens. Legend: F, follicle; GE, glandular epithelium. <i>Scale bar</i> represents 50 µm (the first columnar panels, sense and IgG) and 200 µm (the second columnar panels).