Validation of the gene expression response for four novel IFN-β response genes by real time RT-PCR.

<p>The change in expression levels (2-^ΔΔ<i>C</i>_T) following four hours of IFN-β exposure is shown for four genes that are novel IFN-β response genes. The horizontal bars represent the median values. A: Comparison of the IFN-β response in LCLs from healthy controls (HC, empty symbols, n = 7) and patients with multiple sclerosis (MS, full symbols, n = 20). Fold changes for IFN-β response were significant for both groups (P-value<0.007, Wilcoxon signed rank test). No difference was observed in the IFN-β response between controls and MS LCLs, except for <i>HAPLN3</i>, for which the difference was of a small magnitude (*P-value<0.05, Mann Whitney test). B: The IFN-β response in primary B cells (n = 9) and PBMC (n = 8) from healthy controls and MS patients. All genes displayed a significant response to IFN-β for both B cells and PBMC (P-value<0.001, Wilcoxon signed rank test). Significant differences between the response in B cells and PBMCs is indicated by ** P-value = 0.0003 (Mann Whitney test). The response magnitude of B cells and LCLs differed for all genes, however, these differences were small (P-value<0.03, Mann Whitney test).