WT1 upregulates E-cadherin expression in the presences of Snail.

<p>(A) Immunoblot analysis of E-cadherin and N-cadherin expression in the isogenic SN12C and ACHN cell lines. (B) VHL-knockdown RCC cells were transfected with scrambled or WT1-specifc siRNAs and E-cadherin expression was assessed by immunoblot. (C) Top, HEK293T cells were transfected as indicated and protein expression was assessed by immunoblot. Bottom, analysis of E-cadherin mRNA levels. (D, E) HEK293T (D) or MDCK (E) cells were cotransfected with the indicated plasmids and the <i>CDH1</i> promoter reporter construct, and luciferase activity was measured. Graphs represent mean±SD of one representative experiment. *, <i>P</i><0.05.