MK591 pre-treatment inhibits 5-LO expression in RAW264.7 cells stimulated by LPS and Lipid A.

<p>(A) RAW264.7 cells were pre-treated for 30 minutes with MK591 or vehicle followed by LPS or Lipid A stimulation for 0.5 hr and 4 hr. Expression of 5-LO were evaluated by immunoblotting of RAW264.7 cell lysates. Actin density was quantified as internal control. Representative immunoblots of 5 independent experiments are presented. (B) Quantitative expression is the mean ± S.E.M. of 5 independent experiments. At 0.5 hr, *p<0.05 and ^†p<0.01 compared to LPS-treated cells, ^‡p<0.05 compared to Lipid A-treated cells; as at 4 hr, ^§p<0.05, ^¶p<0.01 and ^||p<0.001 compared to LPS-treated cells, ^@p<0.01 compared to Lipid A-treated cells.