MK591 pre-treatment inhibits RAW264.7 cell proliferation using WST-1 proliferation assay.

<p>RAW264.7 cells were pre-treated for 30 minutes with MK591 or vehicle followed by LPS or Lipid A stimulation for 0.5 hr and 4 hr. Cell viability was analyzed by WST-1 and quantitative assessment of proliferation is presented as the mean ± S.E.M. *p<0.001 compared to non-treated control cells, ^†p<0.001 compared to LPS-treated cells and ^‡p<0.001 compared to Lipid A-treated cells.