Surface expression of tagged T1r2 and T1r3.

<p>A. Surface expression of tagged T1r2 and T1r3 solely in HEK293 cells. HEK293 cells stably expressing tagged T1r2 or tagged T1r3 were labeled with a rabbit anti-c-Myc antibody or rabbit anti-FLAG antibody under non-permeabilized conditions (scale bar = 50 µm). B. Surface expression of tagged T1r3 in COS-7 and CHO cells. Tagged T1r3 were transiently expressed in COS-7 or CHO cells and were labeled with a rabbit anti-FLAG antibody under non-permeabilized conditions (scale bars = 50 µm) C. Tagged T1rs expression in HEK293 cells. The cells were labeled with a rabbit anti-c-Myc antibody or rabbit anti-FLAG antibody under permeabilized conditions (scale bar = 50 µm). D. Surface expression of FH3 with transient CH2 expression. FH3 cells were labeled with a rabbit anti-FLAG antibody under non-permeabilized conditions after 24-h lipofection with CH2 (scale bar = 50 µm). E. Representative flow cytometry histogram data. HEK293 cells were used as a control. F. Flow cytometry quantification of cell surface expression of tagged T1r3 in intact cells. Data are expressed as the mean fluorescence intensity (MFI) ratio of FLAG labeling (MFI [FLAG]) in cells expressing tagged mouse T1r2, T1r3, T1r2/T1r3, human T1R2, T1R3, or T1R2/T1R3 to that in control HEK293 cells. Statistical significance was calculated by ANOVA followed by Tukey test (*: P<0.05). Error bars: SEM (n = 3).