Surface expression of human-mouse T1r3 chimeras.

<p>A. Construction of T1r3 chimeras. The mouse regions are shaded in grey. B. Surface expression of T1r3 chimeras. HEK293 cells expressing chimeras were labeled with rabbit anti-FLAG antibody under non-permeabilized conditions (scale bar = 50 µm). C. Immunoblot analysis of cells expressing chimeras with rabbit anti-FLAG (2.5×10⁴ cells/well). D. Flow cytometry quantification of cell surface expression of chimeras in intact cells. Data are expressed as the MFI ratio of FLAG labeling (MFI [FLAG]) in chimera-expressing cells. Statistical significance was calculated by ANOVA followed by Tukey test (*: P<0.05). Error bars: SEM (n = 3). E. Representative flow cytometry histogram data.