Characteristics of treatment sensitivity and RASSF6 expression in NPC cells and tissues.

<p>(<b>A, B</b>) CNE-2, S18, and S26 cells were treated with various doses of radiation. The viable cells were evaluated using an MTS assay (<b>A</b>); and the abilities of colony formation upon various radiation doses were compared. *<i>P</i><0.05, **<i>P</i><0.01, ANOVA. (<b>C</b>) mRNA expression of RASSF family members in CNE-2, S18, and S26 cells. (<b>D</b>) Western blot analysis of the RASSF6 protein level in CNE-2, S18, and S26 cells; GAPDH was used as a loading control. (<b>E</b>) RASSF6 mRNA expression levels in 18 relapsed NPC tissues (Relapsed) and 25 untreated NPC tissues (Untreated) were determined by qPCR assays. GAPDH and 18S were used as reference genes. <i>P</i><0.05, student's t-test.