SIRT1 overexpression inhibits the expression of muscle atrophy genes.

<p>(A) Relative mRNA levels of <i>MAFBx</i> and <i>MuRF-1</i> atrophy genes in gastrocnemius muscle of WT and Tg-4140 mice fed or fasted for 24 hrs (n = 3–5). (B) Relative mRNA levels of <i>MAFBx</i> and <i>MuRF-1</i> atrophy genes in gastrocnemius muscle of WT and Tg-4140 mice, which underwent mock surgery (control) or were denervated for 3 days (n = 3–5). (C) Relative mRNA levels of <i>FOXO1</i> and <i>FOXO3</i> transcription factors in gastrocnemius muscle of WT and Tg-4140 mice fed or fasted for 24 hrs (n = 3–5). Data are expressed as mean +/− s.e.m. *p<0.05, **p<0.01, ***p<0.001 by two-tailed unpaired Student's t test.