<i>Atwapl1-1wapl2</i> meiocytes exhibit alterations in homologous chromosome pairing.

<p>FISH was conducted on male meiocytes from wild type (A–D) and <i>Atwapl1-1wapl2</i> (E–L) plants using a telomere repeat probe that binds to a region proximal to the centromere of chromosome 1. Two signals are observed at early leptotene (A, E, I). One signal reflecting synapsed chromosomes is observed at late zygotene and pachytene in wild type and some <i>Atwapl1-1wapl2</i> meiocytes (B, C F, G), while two signals are observed in others (J, K). Two closely spaced signals are typically observed at diplotene in wild type and many <i>Atwapl1-1wapl2</i> meiocytes (D, H) with two widely separated signals in others (L). Images shown for <i>Atwapl1-1wapl2</i> represent the most common phenotypes observed at each stage. Size bar = 10 µm.