Expression of rLiTat 1. 5_{H-SUMO-33-426-Strep} by <i>Pichia pastoris</i>.

<p>A. Western blot with anti-His tag antibody; B: Western blot with anti-Strep tag antibody; C: Coomassie stained 12% SDS-PAGE gel; Protein markers (M): ColorPlus Prestained Protein Marker (NEB; A and B) and LMW-SDS protein marker (GE Healthcare; C); lane 1: supernatant of transfected <i>Pichia pastoris</i> M5 after 25 h induction; lane 2: supernatant of transfected <i>Pichia pastoris</i> M5 after 44 h induction; lane 3: Ni-NTA purified recombinant LiTat 1.5; lane 4: 20× concentrated flow-through of Ni-NTA purified supernatant.