Analysis of regulation of MAPKs in HepG2 cells treated with non-cytotoxic AgNPs and Ag⁺.

<p>(A) Cells were treated with a series of concentrations of 10 nm AgNPs, 100 nm AgNPs or Ag⁺ for 24 hours, and the samples were analyzed with dual-phospho-ERK (Thr202/Tyr204) antibody, ERK antibody, dual-phospho-p38 (Thr180/Tyr182) antibody, p38 antibody, dual-phospho-JNK (Thr183/Tyr185) antibody and JNK antibody, respectively, using western blot. β-actin was used for equal loading. The panel shows images representative of three independent experiments. (B, C, D) The intensity of each chemiluminescence protein band was analyzed by ImageJ and normalized to the MAPKpp/β-actin ratio (control = 1), with the data expressed as mean ± S.D. (n = 3), *<i>p</i><0.05.