Dynamics of SIV plasma viral loads (copy Eq/ml) before and during antiretroviral therapy.

<p>Arrows with numbers below X-axis indicate time of initiation of antiretroviral therapy with RT inhibitors PMPA and FTC, and integrase inhibitor L-870812 (weeks 7 p.i.), addition of integrase inhibitor L-900564 (week 22 p.i.), and protease inhibitor Darunavir with boosting agent Ritonavir (week 30 p.i.). Grey shading shows the period of cART and the red dashed area indicates 3 weeks of SAHA therapy initiated 49 weeks post infection. The limit of detection by standard qRT-PCR is 30 copy Eq/ml (solid line) and ultrasensitive qRT-PCR is 15 copy Eq/ml (dashed line) or 4 copy Eq/ml (dotted line) attained when using larger volumes of plasma. Filled symbols show determined values, and open symbols indicate samples that were below the limit of detection for the indicated level of sensitivity. All tested samples were below the assay threshold sensitivity for the relevant assays from week 22 post infection on.