Nematode resistance test for <i>WRKY6</i> overexpression line and mutant.

<p>The resistance of overexpression line of <i>WRKY6</i> along with its knock out mutant compared to wild type plants after infection with <i>H. schachtii</i>. A: Number of male and female nemaodes per cm of root length calculated at 15 dpi setting the wild type as 100%. Different letters indicate significant differences (P<0.05; ANOVA and LSD). The statistical significance was determined by three independent replicates. Values are means ± SE, n = 15. The bar shows standard error for the mean. B: Size of female syncytia and female nematodes at 14 dpi. Ten syncytia were selected randomly from three independent replicates (total = 30) and the size of syncytia and associated female nematodes was determined. Data were analysed for significance difference using ANOVA (P<0.05) and LSD. Values are means ± SE.