NFκB-dependent signaling is responsible for the synergism between IFN γ and dsRNA (dsR).

<p>A) Well differentiated primary HBE cells were treated with 50 ng/ml IFN γ and/or 25 µg/ml dsRNA for 24 hours in the presence or absence of IκB kinase or JAK inhibitor. RNA was isolated followed by qPCR. Solvent Control: black bar; IκB kinase inhibitor: grey bar; JAK inhibitor: light grey bar. $: p<0.05. Inhibitor treatment vs. solvent control. B) Cells were treated with 50 ng/ml IFN γ and/or 25 µg/ml dsRNA for 3 hours. Cellular protein was collected and analyzed by western blot analysis. Actin was used as a loading control. pSTAT1: phosphorylated STAT1. This is the representative image from three replicates.