Effects of CD147 on FA morphology and cytoskeleton organization in HCC cells.

<p>(A) Endogenous vinculin was immunofluorescently stained. Vinculin-mediated FA areas were quantitatively analyzed with Nikon NIS-Elements software. Error bars represent standard errors of the means. ***<i>P</i><0.0001 (Student’s <i>t</i>-test). (B) FA length/width ratios were analyzed with Nikon NIS-Elements software. Error bars represent standard errors of the means. ***<i>P</i><0.0001 (Student’s <i>t</i>-test). (C) FA mean intensity was analyzed with Nikon NIS-Elements software. Error bars represent standard errors of the means. ***<i>P</i><0.0001 (Student’s <i>t</i>-test). (D) Confocal microscopy images of FA and the cytoskeleton in SMMC-7721 and K7721 cells. Scale bar = 20 µm. (E) Cell lysates from K7721 cells expressing WT-CD147 and glycosylation site-mutated CD147 were analyzed by Western blotting with an anti-CD147 antibody. (F) Confocal microscopy analysis demonstrated the cytoskeleton rearrangement and focal adhesion redistribution induced by N-glycan deletion. Cells transfected with WT-CD147 and site-mutated CD147 were stained with rhodamine-phalloidin (red) and anti-vinculin antibody (green). Scale bar = 20 µm.