Intersex morphology.

<p><b>A–B: Ovotestis 1</b>. All-male rainbow trout testis at 136 dpf exposed chronically to 0.01 µg/L and 0.1 µg/L for 76 days. HES. L: lamellae; C: cords of gonocytes (Go); CN: cell nest in different meiosis stages: Le: leptotene; Zy: zygotene; Pa: pachytene. Scale bar = 50 µm. <b>C–D: </b><b>Ovotestis 2</b>. All-male rainbow trout testis at 136 days post fertilization (dpf) exposed chronically to 0.01 µg/L EE2 for 76 days. HES. Scale bar C = 50 µm, insert in C = 25 µm, D = 50 µm. Figure C shows oocytes appearance in an altered testis structure. The insert in Figure C shows the normal testis structure, with gonocytes (Go) organized in cords (C) observed in other areas of this gonad. Figure D shows oocyte appearance in a normal testis structure. PG: primary growth oocytes; TE: altered testicular tissue; FC: follicular cells. <b>E–F: </b><b>Ovotestis 3</b>. All-male rainbow trout testis at 136 dpf exposed chronically to 0.01 µg/L for 76 days. HES. Inserts show the altered testis structure at higher magnification. Dotted line represents the enlarged tissue section. OM: ovarian-like morphology; PG: primary growth oocytes; TE: altered testicular tissue; Sg: spermatogonia, La: lacune; Td: tubule disorganization; Va: vacuolation. Scale bar E = 300 µm, F = 100 µm, inserts = 50 µm.