Activation of inflammasome is not involved in 7KCh-mediated inflammation.

<p>ARPE19 cells were treated with 8 µM 7KCh for 24 hr and the mRNA inductions of the inflammatory markers were measured by qRT-PCR<b>. (a)</b> Measurements (mean ± s.d., <i>n</i> = 3) with and without the siRNA knockdown of NLRP3. NLRP3 knockdown caused a slight induction in all of the inflammatory markers but only VEGF was statistically significant (2.9 to 5.5 fold). <b>(b)</b> Measurements (mean ± s.d., <i>n</i> = 3) with and without t 10 µM Ac-YVAD-CMK (caspase-1 inhibitor). Ac-YVAD-CMK increased IL-6 (18.6 fold to 24.4 fold) but had no statistically significant effect on any of the other markers. *<i>p</i><0.05, two-tailed Student's t-test. <b>(c)</b> Neovessel area measurement (mm²) in anterior chamber of the rat eyes in response to 7KCh-containing implants was determined as previously described (9). Implants containing 7% 7KCh (n = 14) and implants containing 7% 7KCh and 10% YVAD-CMK (w/w) (n = 6) were compared. No statistically significant anti-angiogenic effect was observed by YVAD-CMK.