Protein kinase CK2 and β-catenin do not mediate 7KCh-induced inflammation.

<p>ARPE19 cells were treated with 8 µM 7KCh for 24 hr and the mRNA inductions of the inflammatory markers were measured by qRT-PCR. <b>(a)</b> Measurements (mean ± s.d., <i>n</i> = 3) with and without 5 µM TBB (CK2 inhibitor). TBB treatment caused a slight increase in IL-1β (4.4 fold to 5.9 fold) and a significant increase in IL-6 induction (17.5 fold to 46.6 fold). <b>(b)</b> Measurements (mean ± s.d., <i>n</i> = 3) with and without siRNA knockdown of β-catenin (Wnt signaling). Knockdown of β-catenin had no effect on 7KCh-induced inflammation. *<i>p</i><0.05, two-tailed Student's t-test.