UVA increases CatK expression at both mRNA and protein levels for three consecutive days in human dermal fibroblasts.

<p>Cultured fibroblasts were irradiated with sham and 10/cm² UVA. Cells were harvested on three consecutive days after irradiation for RNA and protein extraction. (A) Total cellular RNA was analyzed by real-time RT-PCR for CatK. (B) Cell lysates were analyzed by Western blot with anti-CatK antibody. The blot was reprobed with anti-GAPDH to confirm equal loading. Bar graphs represent the relative band intensities (mean±SD from three independent experiments). *P<0.05 vs. untreated control, §P<0.05 vs. UVA-treated groups 24 h and 72 h after irradiation.