Aurora kinase inhibitors promote changes in Aurora kinase activity and induce cleavage of apoptotic markers in leukemia cell lines C1 and KOPN8.

<p>C1 and KOPN8 were treated with Aurora kinase inhibitors or vehicle control (DMSO) at 1 µM for 18 hours. The majority of the inhibitors tested induced dephosphorylation of one or more of the Aurora kinases, with the exception of Aurora-A Inhibitor I and ENMD-2076. Evidence of apoptosis is shown with increased cleavage of PARP and caspase 7 compared to vehicle control in all of the inhibitors tested, with the exception of JNJ-7706621 for both C1 and KOPN8 and ENMD-2076 for KOPN8. β-Actin was used as a loading control. Data presented are representative of three separate experiments.